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KMID : 1098420040120020123
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Korean Journal of Medicinal Crop Science
2004 Volume.12 No. 2 p.123 ~ p.128
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Growth Pattern and Content of Tropane Alkaloids of Metabolic Engineered Scopolia parviflora Hairy Root Lines
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Kang Young-Min
Min Ji-Yun
KIm Won-Jung
Kim Yong-Duck
Lee Byung-Hyun
Choi Myung-Suk
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Abstract
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Hyoscyamine and scopolamine are two most common tropane alkaloids found in the Solanaceae. The pEB expression vector carrying Nspmt gene was transformed to Agrobacterium rhizogenes. The growth of transgenic hairy roots were approximately to 80% of the wild type root. Transgenic hairy roots are less developed on only their branch roots than those of the wild type root. The extracts from Nspmt transgenic hairy root lines, 3 and 5 contained between 3.52 and 4.23 mg/g dry weight as hyoscyamine and did between 5.23 to 6.40 mg/g dry weight as scopolamine. These results showed that the overexpression of the pmt gene enhanced tropane alkaloids production of S. parviflora transformed roots and this improvement affected both hyoscyamine and scopolamine production.
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KEYWORD
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root growth index, morphology, metabolic engineering, tropane alkaloids
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